Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: ATG16L1 Regulates Reparative Function of Peritoneal Macrophages During Acute Drug-induced Liver Injury

doi: 10.1016/j.jcmgh.2025.101674

Figure Lengend Snippet: ATG16L1 in PMs promotes hepatocyte proliferation via the IL-10-CXCR2 axis. PMs were injected into APAP-treated WT recipient mice. Liver tissues were collected at 24 and 48 hours post DILI. IHC for PCNA ( A ). WB ( B ) and qPCR ( C, D ) for CXCR2 and PCNA expression in liver tissues at 48 hours post DILI. Primary hepatocytes were co-cultured with PMs stimulated with HA. qPCR for CXCR2 and PCNA in hepatocytes ( E, F ). PMs were stimulated by HA combined with NAC or DC-LC3in-D5. IL-10 levels were assessed by qPCR and ELISA ( G–J ). IL-10 neutralizing antibody was added into the co-cultured primary hepatocytes and PMs stimulated with HA. Expression of CXCR2 and PCNA in hepatocytes ( K–M ). (n = 6/group). Data are presented as mean ± SEM. Each point represents an independent experiment. ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001.

Article Snippet: Rabbit anti-mouse ATG16L1 (1:1000, ab187671, Abcam), rabbit anti-mouse CD44 (1:1000, ab243894, Abcam), rabbit anti-mouse OAS3 (1:1000, 21915-1-AP, proteintech), rabbit anti-mouse SLFN5 (1:1000, AF15102, AIFang biological), rabbit anti-mouse DHX58 (1:1000, 11355-1-AP, proteintech), rabbit anti-mouse EIF2AK2 (1:5000, 18244-1-AP, proteintech), rabbit anti-mouse TRIM21 (1:5000, 12108-1-AP, proteintech), rabbit anti-mouse MerTK (1:1000, 27900-1-AP, proteintech), rabbit anti-mouse Axl (1:1000, ab215205, Abcam), rabbit anti-mouse TYRO3 (1:1000, 28513-1-AP, proteintech), rabbit anti-mouse TIM4 (1:1000, ab47637, Abcam), rabbit anti-mouse CXCR2 (1:1000, 20634-1-AP, proteintech), rabbit anti-mouse LC3 (1:1000, ab192890, Abcam), rabbit anti-mouse p62 (1:1000, ab109012, Abcam), rabbit anti-mouse PCNA (1:1000, ab29, Abcam), and mouse anti-mouse β-actin (1:1000, #3700S, Cell Signaling Technology) were used.

Techniques: Injection, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: medRxiv

Article Title: Altered neutrophil G-protein receptor signalling linked to impaired chemotaxis and increased ROS and NET production in older people with frailty

doi: 10.64898/2025.12.16.25342352

Figure Lengend Snippet: (A) Expression of CXCR1 (CD181), CXCR2 (CD182) and CD177 via flow cytometry. (B) Removal of the RA group resulted in significant differences. (C) Chemotaxis of neutrophils towards IL-8, fMLP or random migration (UT). (D) ERK-mediated signalling network predicted to be regulating chemotaxis in FR neutrophils (adj. p=5.23×10 -9 ). Red = up-regulated gene expression, Orange = predicted activation. Representative western blots and densitometry (n=4) for (E) phosphorylated ERK (ERK-P) in untreated (UT) and 5 min IL-8 treated neutrophils and (F) phosphorylated RAC-1 (RAC1-P) in freshly isolated, 0h neutrophils. Purple = FR (n=4-10), Pink = RA (n=4-9), Orange = HO (n=4-10), Green = HY (n=4-7). Analysed by ANOVA (*p<0.05, **p<0.01, ***p<0.001).

Article Snippet: Antibodies used were CD177 FITC Monoclonal Antibody (MEM-166, Thermo Fisher), CD54 (ICAM-1) PE-Vio615, REAfinity antibody (Miltenyi), CD181 (CXCR1) FITC REAfinity antibody (Miltenyi), CD182 (CXCR2) APC, REAfinity antibody (Miltenyi), REAfinity isotype controls (APC, PE-Vio615, FITC, Miltenyi) or unstained (US) controls.

Techniques: Expressing, Flow Cytometry, Chemotaxis Assay, Migration, Gene Expression, Activation Assay, Western Blot, Isolation

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: Inflammatory cell infiltration is significantly higher in PVNS synovium compared to OA synovium. ( A ) Proportion of all 22 immune infiltration cells based on CIBERSORT in the dataset GSE3698 . ( B ) Boxplots visualize the differences of all 22 immune cells calculated by CIBERSORT in OA, RA and PVNS tissues in GSE3698 . ( C and D ) Immunohistochemistry image for M2 macrophages (CD206; C ) and Neutrophils (CXCR2; D ) in the synovium of the patients with OA and PVNS. Scale bar: 50µm (20X) or 20um (40X). *P < 0.05; **P < 0.01.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Immunohistochemistry