Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: Identification of the key genes expression in external datasets. A IFIT5, IFI16, TRIM22, IFI44L, IRF9, OAS3, BST2, CASP1, CXCR2 were statistically significant in AF expression profile ( GSE41177 ). B IFIT5、CASP1 and CXCR2 were statistically significant in COPD expression profile ( GSE56766 ). C - D ROC of IFIT5、CASP1 and CXCR2 in the AF( GSE79768 ) dataset. -, not significant.;*, p <0.05; **, p <0.01; ***, p < 0.001

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques: Expressing

Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: Validation of CASP1, CXCR2, and IFIT5 expression in different clinical groups at mRNA and protein levels. A – C Relative mRNA expression levels of CASP1, IFIT5, and CXCR2 were assessed by qRT-PCR in peripheral blood samples from healthy controls (CON), atrial fibrillation (AF), chronic obstructive pulmonary disease (COPD), and AF combined with COPD (AF+COPD). D – F Quantification of CASP1, IFIT5, and CXCR2 protein expression levels based on Western blot analysis. Signal intensities were normalized to GAPDH. G – I Serum protein concentrations of CASP1 (pmol/L), IFIT5 (pg/mL), and CXCR2 (ng/mL) measured by ELISA in the four groups. J Representative Western blot images for CASP1, IFIT5, and CXCR2 with GAPDH as loading control. *, p < 0.05; **, p <0.01; ***, p < 0.001

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Journal: BMC Medical Genomics

Article Title: Shared molecular signatures between atrial fibrillation and chronic obstructive pulmonary disease: an integrated bioinformatic analysis with experimental validation

doi: 10.1186/s12920-026-02335-4

Figure Lengend Snippet: A Transcription factor (TF)-gene interaction network based on key genes. B miRNA-gene interaction network based on key genes. C , E , G Overall docking models of CASP1, CXCR2, and IFIT5 proteins with estradiol, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes. D , F , H Overall docking models of CASP1, CXCR2, and IFIT5 proteins with methyl methanesulfonate, along with two-dimensional and three-dimensional spatial interaction diagrams of the ligand-protein complexes

Article Snippet: Proteins were then transferred onto the membranes using a constant current of 400 mA for 0.5 h. Following transfer, the membranes were incubated overnight at 4 °C with primary antibodies specific for CASP1 (WL03450, Wanleibio), CXCR2 (bs-1629R, Bioss), IFIT5 (CAP4911, Cohesionbio), and GAPDH (10494-1-AP, Proteintech), ensuring specific detection of target proteins.

Techniques:

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Multi-omics study on tumor-associated macrophages remodeling the tumor microenvironment via the CXCL5-CXCR2 axis to drive immune escape in bladder cancer

doi: 10.1007/s00262-026-04353-8

Figure Lengend Snippet: Tumor associated macrophages promote immune escape of bladder cancer cell through the CXCL5-CXCR2-NF-κB-PD-L1 pathway. A The establishment process of co-culture model by using macrophage and tumor cell. B The establishment process of co-culture model by using macrophage, tumor cell and activated Jurkat T cell. C The RT-qPCR results of chemokine changes in M0 macrophage cocultured with T24. D The RT-qPCR results of chemokine changes in T24 co-cultured with M0 macrophage. E CXCL5-CXCR2-NF-κB-PD-L1 pathway related protein and IDO1 protein were increased in macrophage co-cultured with bladder cancer cell. F The heatmap of tryptophan metabolomics. G The concentration of INF-γ, IL4, IL2, IL6 and TNF-α in conditioned medium derived from the co-culture system of Jurkat T cell and macrophage. H CXCL5-CXCR2-NF-κB-PD-L1 pathway related protein were increased in bladder cancer cell co-cultured with macrophage. I The concentration of INF-γ, IL4 and IL2 in conditioned medium derived from the co-culture system of Jurkat T cell and T24 cell. J The concentration of INF-γ, IL4 and IL2 in conditioned medium derived from the co-culture system of Jurkat T cell and 5637 cell. ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: CXCR2 , Rabbit monoclonal , Proteintech , 1:1000.

Techniques: Co-Culture Assay, Quantitative RT-PCR, Cell Culture, Concentration Assay, Derivative Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Multi-omics study on tumor-associated macrophages remodeling the tumor microenvironment via the CXCL5-CXCR2 axis to drive immune escape in bladder cancer

doi: 10.1007/s00262-026-04353-8

Figure Lengend Snippet: The expression level of CXCL5 is positively correlated with the expression of PD-L1 and enhancing the efficacy of immunotherapy in bladder cancer. A The correlation between CXCL5 and PD-L1 in TCGA-BLAC. B The expression of CXCL5 in bladder cancer patients with different expression of PD-L1 based on IMvigor210 cohort. C The change of PD-L1 RNA level in CXCL5 knockdown T24 cell line. D The change of PD-L1 RNA level in CXCL5 knockdown MB49 cell line E CXCL5-CXCR2-NF-κB-PD-L1 pathway related protein expression in CXCL5 knockdown cell line. F The establishment process of C57 immunotherapy model. G The image of C57 subcutaneous tumors according to different group. H The tumor weight of subcutaneous tumors according to different group. ** P < 0.01, **** P < 0.0001

Article Snippet: CXCR2 , Rabbit monoclonal , Proteintech , 1:1000.

Techniques: Expressing, Knockdown

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Multi-omics study on tumor-associated macrophages remodeling the tumor microenvironment via the CXCL5-CXCR2 axis to drive immune escape in bladder cancer

doi: 10.1007/s00262-026-04353-8

Figure Lengend Snippet: A schematic diagram of CXCL5-CXCR2 axis mediated the crosstalk between bladder cancer cell and macrophage

Article Snippet: CXCR2 , Rabbit monoclonal , Proteintech , 1:1000.

Techniques: